Kelstrup C.D., Jersie-Christensen R.R., Batth T.S., Arrey T.N., Kuehn A., Kellmann M., Olsen J.V. Libraries were mapped to the hg38 transcriptome (UCSC_knownGene) and subsequent analysis was performed using a custom script.

Here, we have explored the function of the human orphan MTase ZCCHC4. Classification of all DNA methyltransferases, Novel N-terminal and lysine methyltransferases that target translation elongation factor 1A in yeast and human, Molecular basis of Pirh2-mediated p53 ubiquitylation, DHHC palmitoyl transferases: substrate interactions and (patho)physiology, Fatty acyl recognition and transfer by an integral membrane S-acyltransferase, NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47, Identification of a selective polymerase enables detection of N(6)-methyladenosine in RNA, Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome, N(6)-Methyladenine hinders RNA- and DNA-directed DNA synthesis: application in human rRNA methylation analysis of clinical specimens, Identification of sites of 2′-O-methylation vulnerability in human ribosomal RNAs by systematic mapping, The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112, N(6-)Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation, RNA-guided isomerization of uridine to pseudouridine–pseudouridylation, Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells, SAF-A/hnRNP-U localization in interphase and metaphase, Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling, Codon optimality is a major determinant of mRNA stability, Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation, An integrated, structure- and energy-based view of the genetic code, Codon optimality, bias and usage in translation and mRNA decay, An elaborate pathway required for Ras-mediated epigenetic silencing, Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors, Optimized E. coli expression strain LOBSTR eliminates common contaminants from His-tag purification. Ribosome profiling libraries of ZCCHC4-deficient HAP-1 cells were generated in triplicates and WT HAP-1 cells were generated in duplicates. Interactions involving only G or C in the first two nucleotides of the codon are considered ‘STRONG’, those involving only A or T are considered ‘WEAK’, whereas the remaining ones are classified as ‘INTERMEDIATE’ (Figure 6B). m6A4220 (green) is shown on the 28S rRNA structure, together with other known, snoRNA-independent methyl modifications (m1A1322, purple; m3C3782, blue; Gm4196, olive; m5C4447, pink; Um4498, cyan; Gm4499, yellow; m3U4530, red). found only ∼55% methylation in HepG2 cells. Microbial growth rates may be limited by catalytic rates or by the rate of diffusion of resources, which are often supplied with some irregularity in the natural environment. Yes

METTL16 was recently discovered as a novel MTase that introduces m6A in a stem–loop structure in the U6 spliceosomal RNA, but also modifies similar structures in various mRNAs, thereby regulating their function (18,19). Schwartz S., Mumbach M.R., Jovanovic M., Wang T., Maciag K., Bushkin G.G., Mertins P., Ter-Ovanesyan D., Habib N., Cacchiarelli D. et al. A discussion on the validity of this assumption can be found elsewhere [6]. Wild-type recombinant ZCCHC4, or a putatively enzymatically inactive mutant (D276A) was incubated with a 5′-biotinylated, non-methylated RNA-oligonucleotide encompassing the A4220-containing stem–loop (representing nucleotides 4204 – 4228 in 28S rRNA) in the presence of [3H]AdoMet. Funding acquisition, Jia G., Fu Y., Zhao X., Dai Q., Zheng G., Yang Y., Yi C., Lindahl T., Pan T., Yang Y.G. In glucose fed batch cultures and chemostats across a range of dilution rates, transporter abundances qualitatively matched neither of the two previous model predictions (Fig 1).

The vertical green dashed line represents the critical substrate concentration S*. Fig 5 shows contours of uptake rates in the plane of transporter abundance and glucose concentrations. Department of Oceanography, University of Hawai‘i at Mānoa, Honolulu, Hawai‘i, United States of America, Roles where is the effective half-saturation concentration at the porter limit, which is independent of the number of transporters, The closely related YTHDF1, YTHDF2 and YTHDF3 are localized to the cytosol where they promote translation and turnover of m6A containing mRNAs (12,13,22,23).

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